Práce byla zaměřena na obecnou charakteristiku enzymů, jejich katalytickou aktivitu a používané metodiky pro stanovení aktivity převážně lipáz. Nejpoužívanějšími metodami pro stanovení aktivity jsou fotometrická metoda, kalorimetrická metoda, metoda o-ftalátdialdehydová a difůzní metoda.
Annotation in English
The work was tocused on the generally characteristic of enzymes, the enzymes activity and methods for a determination of lipases activity. The most used methods for a determination of enzymes activity are photometric method, calorimetric method, o-phthaldialdehyde method and diffusion method.
Práce byla zaměřena na obecnou charakteristiku enzymů, jejich katalytickou aktivitu a používané metodiky pro stanovení aktivity převážně lipáz. Nejpoužívanějšími metodami pro stanovení aktivity jsou fotometrická metoda, kalorimetrická metoda, metoda o-ftalátdialdehydová a difůzní metoda.
Annotation in English
The work was tocused on the generally characteristic of enzymes, the enzymes activity and methods for a determination of lipases activity. The most used methods for a determination of enzymes activity are photometric method, calorimetric method, o-phthaldialdehyde method and diffusion method.
Popište používané metodiky ke stanovení aktivity enzymů.
Recommended resources
[1] JANDAL,J.M. Some factors affecting lipase activity in goat milk, Small Ruminant Research 16, 1995.
[2] ANTONELLI, M.L. a kol. Determination of free fatty acids and lipase activity in milk:quality and storage markers, Talanta 58, 2002.
[3] HEDEGAARD, R.V. a kol. Comparison of desriptive sensory analysis and chemical analysis for oxidative changes in milk, Amarican Dairy Science Association, 2006.
[4] ETZEL, M.R. Enzyme inactivation in a droplet forming a bubble during drying, Journal of Food Engineering 27, 1996.
Recommended resources
[1] JANDAL,J.M. Some factors affecting lipase activity in goat milk, Small Ruminant Research 16, 1995.
[2] ANTONELLI, M.L. a kol. Determination of free fatty acids and lipase activity in milk:quality and storage markers, Talanta 58, 2002.
[3] HEDEGAARD, R.V. a kol. Comparison of desriptive sensory analysis and chemical analysis for oxidative changes in milk, Amarican Dairy Science Association, 2006.
[4] ETZEL, M.R. Enzyme inactivation in a droplet forming a bubble during drying, Journal of Food Engineering 27, 1996.