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Lecturer(s)
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Schrammová Marie, Ing.
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Víchová Zdenka, doc. Ing. Ph.D.
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Skopalová Kateřina, Ing. Ph.D.
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Course content
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- 1. Introduction to the cell laboratory and work safety. - 2. Work in an aseptic environment and aseptic techniques. - 3.-4. Basics of working with cell and tissue cultures. - 5.-7. Cell viability quantification. - 8. DNA damage by oxidative stress. - 9-10 Evaluation of cell adhesion, proliferation, and differentiation using fluorescence microscopy. - 11.-13. Cultivation within scaffolds and evaluation of cell ingrowth into scaffolds using bioreactors. - 14. Eventual replacement of the laboratory task and awarding of classification credits.
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Learning activities and teaching methods
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Simple experiments, Practice exercises, Individual work of students
- Preparation for course credit
- 120 hours per semester
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| learning outcomes |
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| Knowledge |
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| explain the basic terms related to cell culture |
| explain the basic terms related to cell culture |
| distinguish the basic morphological features of cell cultures |
| distinguish the basic morphological features of cell cultures |
| clarify the requirements for biocompatibility testing |
| clarify the requirements for biocompatibility testing |
| assess the legislative aspects of biocompatibility testing |
| assess the legislative aspects of biocompatibility testing |
| classify and characterize the cytotoxicity of materials |
| classify and characterize the cytotoxicity of materials |
| Skills |
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| design a comprehensive solution for determining biocompatibility |
| design a comprehensive solution for determining biocompatibility |
| perform basic cytotoxicity tests |
| perform basic cytotoxicity tests |
| practically evaluate cell viability |
| practically evaluate cell viability |
| assess cell morphology |
| assess cell morphology |
| operate devices used for basic characterization of cell properties |
| operate devices used for basic characterization of cell properties |
| teaching methods |
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| Knowledge |
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| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Skills |
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| Simple experiments |
| Simple experiments |
| Individual work of students |
| Individual work of students |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Analysis of the student's performance |
| Analysis of the student's performance |
| Analysis of works made by the student (Technical products) |
| Analysis of works made by the student (Technical products) |
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Recommended literature
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ALBERTS, B. et al. Molecular Biology of the Cell. 5th Ed.. Garland Science.
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COLLINS, F.S. Řeč života: DNA a revoluce v personalizované medicíně. Praha: Academia, Galileo, 2012. ISBN 978-80-200-2161-8.
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FRESHNEY, R. I. Culture of animal cells: a manual of basic technique. 2005. ISBN 0471453293.
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KUCIEL, J., URBAN, T. Principy genetiky. Brno: MU Brno, 2016. ISBN 978-80-7509-385-1.
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ROSYPAL, S. Nový přehled biologie. . Praha: Scientia, 2003. ISBN 80-7183-268-5.
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Snustad, D. Peter. Genetika. Vyd. 1. Brno : Masarykova univerzita, 2009. ISBN 978-80-210-4852-2.
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