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Lecturer(s)
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Buňková Leona, prof. RNDr. Ph.D.
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Course content
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- Introduction to the study of molecular biology. Genome of prokaryotes and eukaryotes. - Nucleic acid - carrier of genetic information. - Proteins - structure, properties, meaning. - Expression of genetic information - replication, transcription and translation. - Regulation of gene expression. - Changes in genetic information - cause, essence, consequences. - Genetic engineering and gene cloning. - Genetically modified organisms and the relationship to the environment. Legislative regulations in relation to GMOs. - Possibilities of applying genetically modified organisms in environmental protection, agriculture, food industry and pharmacy. - Bioethics - the problem of interspecies gene transfer and preparation of transgenic organisms. - Molecular biological methods and their use I (DNA isolation, ELFO, hybridization). - Molecular biological methods and their use II (PCR, restriction, RFLP, proteins). - Molecular biological methods and their use III (DGGE, TGGE, sequencing, genomic libraries). - Bioinformatics - genome analysis (identification; searching for characteristic sequences), design of primers for PCR/real time-PCR, statistical methods of data evaluation.
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Learning activities and teaching methods
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Methods for working with texts (Textbook, book), Individual work of students
- Preparation for examination
- 50 hours per semester
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| learning outcomes |
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| Knowledge |
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| characterise the structure of biomacromolecules - nucleic acids and proteins |
| characterise the structure of biomacromolecules - nucleic acids and proteins |
| describe the expression of genetic information |
| describe the expression of genetic information |
| explain the nature of mutagenesis, genome analysis and the principle of gene libraries |
| explain the nature of mutagenesis, genome analysis and the principle of gene libraries |
| describe mechanisms of genetic information change (mutation, transposition, conjugation, transformation, transduction) |
| describe mechanisms of genetic information change (mutation, transposition, conjugation, transformation, transduction) |
| explain the use of molecular biology methods |
| explain the use of molecular biology methods |
| Skills |
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| critically assess the use of individual molecular biological methods |
| critically assess the use of individual molecular biological methods |
| apply various methods of DNA isolation, interpret and evaluate the results and determine the quality, purity and concentration of the DNA obtained |
| apply various methods of DNA isolation, interpret and evaluate the results and determine the quality, purity and concentration of the DNA obtained |
| design a primer sequence and perform DNA amplification by PCR |
| design a primer sequence and perform DNA amplification by PCR |
| use appropriate restriction enzymes depending on the intended DNA manipulation, apply restriction analysis and evaluate the results |
| use appropriate restriction enzymes depending on the intended DNA manipulation, apply restriction analysis and evaluate the results |
| analyze DNA sequencing results |
| analyze DNA sequencing results |
| teaching methods |
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| Knowledge |
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| Methods for working with texts (Textbook, book) |
| Individual work of students |
| Individual work of students |
| Methods for working with texts (Textbook, book) |
| Skills |
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| Individual work of students |
| Individual work of students |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Oral examination |
| Oral examination |
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Recommended literature
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Alberts, B. et al. Molecular Biology of the Cell. New York: Garland Science, 2002.
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ALBERTS, B. Základy buněčné biologie. Ústí nad Labem: Espero, 2001. ISBN 80-902906-2-0.
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CRAIG, N.L. Molecular Biology: Principles of Genome Function. 2nd Ed.. Oxford University Press. ISBN 978-0-19-965857-2.
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GLICK B.R., PASTERNAK J.J. Molecular Biotechnology: Principles and Applications of Recombinant DNA. ASM Press, Washington, D.C., 1994.
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HARZEVILI, F.D., CHEN, H. Microbial Biotechnology: Progress and Trends. Boca Raton: CRC Press, 2015.
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LEHNINGER A., NELSON D.L., YOUNG P. Principles of Biochemistry. W.H. Freeman & Company, 2007.
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SNUSTAD, D.P., SIMMONS, M.J., RELICHOVÁ, J. et al. Genetika. Brno: Masarykova univerzita, 2009.
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ŠMARDA, J. Metody molekulární biologie. Brno: MU Brno, 2005. ISBN 80-210-3841-1.
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