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Lecturer(s)
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Koutný Marek, prof. Mgr. Ph.D.
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Course content
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- Classical biotechnology, biotechnology division, fermentation processes. Benefits of biotechnology. - Theoretical foundations of molecular biotechnology. Variability of DNA, genetic polymorphism. Genome, transcriptome, metabolom, proteome. Organisms used in biotechnology. Tissue culture. - Recombinant DNA technology. Selection of cells with the cloned gene. Genomic library. - Cloning and expression vectors - types and use, design, optimization of expression, the factors influencing the expression of foreign organisms. Cloning by recombination. - In vitro mutagenesis, random mutagenesis, targeted changes in genetic material, mutagenesis using mutagenic oligonucleotides. Synthesis genes. - Cloning and expression of genes in prokaryotic organisms, and usability. Strains of Escherichia coli in molecular biology. Extremophiles prokaryotic microorganisms and their use in biotechnology. - Methods of gene transfer into eukaryotic organisms (transfection, electroporation, microinjection, vector systems). Cloning and expression of genes in yeast, plant and animal cells. - Protein Engineering, Heterologous protein production in foreign cells, factors affecting the yield of native protein. - Use of enzymes in molecular biotechnology, reaction kinetics. Immobilized systems. - Genetically modified organisms and their applications. GMO Legislation in Relation to the EU. Bioethics - the problem of interspecies gene transfer and preparation of transgenic organisms. - Molecular Biotechnology in the food industry - the use of genetically modified bacteria, yeasts and fungi in food production. Health risks. Detection of genetically modified organisms in food. Immobilized biosystems in the food. - Production of antibodies, therapeutic proteins and vaccines. Nucleic acids as therapeutics. Production of pharmacologically important compounds, biopolymers and materials with new properties in unrelated hosts. - Biodetoxikace, biosorption of biotechnology, microbial insecticides. Other industries using molecular biotechnology. - Nanobiotechnology - nanoparticles used for labeling substances, immunomagnetic separation. S-layers and their applications in nanotechnology, nanopores.
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Learning activities and teaching methods
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Methods for working with texts (Textbook, book), Individual work of students
- Preparation for examination
- 50 hours per semester
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| learning outcomes |
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| Knowledge |
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| Student has advanced knowledge in the molecular biotechnology. |
| Student has advanced knowledge in the molecular biotechnology. |
| teaching methods |
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| Individual work of students |
| Individual work of students |
| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| Skills |
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| Individual work of students |
| Individual work of students |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Oral examination |
| Oral examination |
| Analysis of seminar paper |
| Analysis of seminar paper |
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Recommended literature
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ALBERTS B. a kol. Základy buněčné biologie. Espero Publishing, 2001.
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ALBERTS B. a kol. Základy buněčné biologie. Espero Publishing, Ústí nad Labem, 2004.
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ALBERTS B. et al. Molecular Biology of the Cell. Garland Science, New York, 2002.
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GLICK, B.J., PASTERNAK, J.J., PATTEN, C.L. Molecular biotechnology: Principles and applications of recombinant DNA. ASM Press, 2010.
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Glick B.J., Paternak J.J., Patten C.L. Molecular biotechnology: Principles and applications of recombinant DNA. 2010.
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Heller K.J. Genetically engineered food. Methods and detection and genomics. 2006.
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LODISH H. et al. Molecular Cell Biology. W. H. Freeman and Company, 2000.
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Niemeyer C, Mirkin C. Nanobiotechnology. 2004.
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ROSYPAL S. Úvod do molekulární biologie. Brno, 1997.
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Sambrook J., Russell D.W. Molecular Cloning: A laboratory manual. 2001.
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Twynman R., Primrose S.B. Principles of gene manipulation and genomics. 2006.
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Wink M. An introduction to molecular biotechnology. Molecular fundamentals, methods and applications in modeern biotechnology. 2006.
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