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Lecturer(s)
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Koutný Marek, prof. Mgr. Ph.D.
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Course content
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- Classical biotechnology, biotechnology division, fermentation processes. Benefits of biotechnology. - Theoretical foundations of molecular biotechnology. Variability of DNA, genetic polymorphism. Genome, transcriptome, metabolom, proteome. Organisms used in biotechnology. Tissue culture. - Recombinant DNA technology. Selection of cells with the cloned gene. Genomic library. - Cloning and expression vectors - types and use, design, optimization of expression, the factors influencing the expression of foreign organisms. Cloning by recombination. - In vitro mutagenesis, random mutagenesis, targeted changes in genetic material, mutagenesis using mutagenic oligonucleotides. Synthesis genes. - Cloning and expression of genes in prokaryotic organisms, and usability. Strains of Escherichia coli in molecular biology. Extremophiles prokaryotic microorganisms and their use in biotechnology. - Methods of gene transfer into eukaryotic organisms (transfection, electroporation, microinjection, vector systems). Cloning and expression of genes in yeast, plant and animal cells. - Protein Engineering, Heterologous protein production in foreign cells, factors affecting the yield of native protein. - Use of enzymes in molecular biotechnology, reaction kinetics. Immobilized systems. - Genetically modified organisms and their applications. GMO Legislation in Relation to the EU. Bioethics - the problem of interspecies gene transfer and preparation of transgenic organisms. - Molecular Biotechnology in the food industry - the use of genetically modified bacteria, yeasts and fungi in food production. Health risks. Detection of genetically modified organisms in food. Immobilized biosystems in the food. - Production of antibodies, therapeutic proteins and vaccines. Nucleic acids as therapeutics. Production of pharmacologically important compounds, biopolymers and materials with new properties in unrelated hosts. - Biodetoxikace, biosorption of biotechnology, microbial insecticides. Other industries using molecular biotechnology. - Nanobiotechnology - nanoparticles used for labeling substances, immunomagnetic separation. S-layers and their applications in nanotechnology, nanopores.
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Learning activities and teaching methods
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Methods for working with texts (Textbook, book), Individual work of students
- Preparation for examination
- 50 hours per semester
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| learning outcomes |
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| Knowledge |
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| Student has advanced knowledge in the molecular biotechnology. |
| Student has advanced knowledge in the molecular biotechnology. |
| The knowledge of gene editing technologies is essential for understanding tools like CRISPR-Cas9, which allow for precise modifications to DNA, enabling advancements in genetic engineering, therapeutic development, and agricultural biotechnology. |
| The knowledge of gene editing technologies is essential for understanding tools like CRISPR-Cas9, which allow for precise modifications to DNA, enabling advancements in genetic engineering, therapeutic development, and agricultural biotechnology. |
| The knowledge of recombinant DNA technology is crucial for recognizing how DNA from different sources can be combined to create genetically modified organisms (GMOs), produce pharmaceuticals like insulin, and develop innovative treatments. |
| The knowledge of recombinant DNA technology is crucial for recognizing how DNA from different sources can be combined to create genetically modified organisms (GMOs), produce pharmaceuticals like insulin, and develop innovative treatments. |
| The knowledge of synthetic biology is vital for understanding how biological systems and organisms can be engineered from the ground up, allowing for the creation of new biological parts, devices, and systems for various applications. |
| The knowledge of synthetic biology is vital for understanding how biological systems and organisms can be engineered from the ground up, allowing for the creation of new biological parts, devices, and systems for various applications. |
| The knowledge of protein engineering is important for designing and optimizing proteins with novel or enhanced functions, which can be used in industrial processes, drug development, and therapeutic applications. |
| The knowledge of protein engineering is important for designing and optimizing proteins with novel or enhanced functions, which can be used in industrial processes, drug development, and therapeutic applications. |
| The knowledge of high-throughput sequencing technologies is necessary for understanding how rapid sequencing of DNA and RNA enables large-scale genomic studies, personalized medicine, and advanced research in genomics and transcriptomics. |
| The knowledge of high-throughput sequencing technologies is necessary for understanding how rapid sequencing of DNA and RNA enables large-scale genomic studies, personalized medicine, and advanced research in genomics and transcriptomics. |
| Skills |
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| The competency to perform literature searches and reviews to stay updated with the latest advancements and methodologies in molecular biotechnology, enabling informed research and development decisions. |
| The competency to perform literature searches and reviews to stay updated with the latest advancements and methodologies in molecular biotechnology, enabling informed research and development decisions. |
| The competency to use bioinformatics tools and databases for analyzing genetic and protein sequences, understanding gene function, and identifying potential targets for genetic engineering or therapeutic interventions. |
| The competency to use bioinformatics tools and databases for analyzing genetic and protein sequences, understanding gene function, and identifying potential targets for genetic engineering or therapeutic interventions. |
| The competency to design and simulate molecular biology experiments using in-silico methods, including the creation of plasmid maps, prediction of gene editing outcomes, and modeling of protein structures. |
| The competency to design and simulate molecular biology experiments using in-silico methods, including the creation of plasmid maps, prediction of gene editing outcomes, and modeling of protein structures. |
| The competency to analyze high-throughput sequencing data using software tools to interpret genomic, transcriptomic, and proteomic data, facilitating discoveries in gene function, regulation, and expression. |
| The competency to analyze high-throughput sequencing data using software tools to interpret genomic, transcriptomic, and proteomic data, facilitating discoveries in gene function, regulation, and expression. |
| The competency to critically evaluate scientific literature to assess the validity and reliability of experimental methods and results in molecular biotechnology, enabling the development of evidence-based research projects and innovations. |
| The competency to critically evaluate scientific literature to assess the validity and reliability of experimental methods and results in molecular biotechnology, enabling the development of evidence-based research projects and innovations. |
| teaching methods |
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| Knowledge |
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| Individual work of students |
| Individual work of students |
| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| Skills |
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| Individual work of students |
| Individual work of students |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Oral examination |
| Oral examination |
| Analysis of seminar paper |
| Analysis of seminar paper |
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Recommended literature
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ALBERTS B. a kol. Základy buněčné biologie. Espero Publishing, 2001.
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ALBERTS B. a kol. Základy buněčné biologie. Espero Publishing, Ústí nad Labem, 2004.
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ALBERTS B. et al. Molecular Biology of the Cell. Garland Science, New York, 2002.
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GLICK, B.J., PASTERNAK, J.J., PATTEN, C.L. Molecular biotechnology: Principles and applications of recombinant DNA. ASM Press, 2010.
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Glick B.J., Paternak J.J., Patten C.L. Molecular biotechnology: Principles and applications of recombinant DNA. 2010.
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Heller K.J. Genetically engineered food. Methods and detection and genomics. 2006.
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LODISH H. et al. Molecular Cell Biology. W. H. Freeman and Company, 2000.
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Niemeyer C, Mirkin C. Nanobiotechnology. 2004.
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ROSYPAL S. Úvod do molekulární biologie. Brno, 1997.
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Sambrook J., Russell D.W. Molecular Cloning: A laboratory manual. 2001.
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Twynman R., Primrose S.B. Principles of gene manipulation and genomics. 2006.
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Wink M. An introduction to molecular biotechnology. Molecular fundamentals, methods and applications in modeern biotechnology. 2006.
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