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Lecturer(s)
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Janalíková Magda, doc. Mgr. Ph.D.
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Pleva Pavel, Ing. Ph.D.
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Koutný Marek, prof. Mgr. Ph.D.
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Máčalová Daniela, Ing.
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Riemel Jakub, Ing.
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Course content
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1. The significance and role of molecular biology within related disciplines. Basic concepts. 2. The cell as a functional system: organization of prokaryotic and eukaryotic cells in the context of genetic information flow. 3. Nucleic acids: structure, function, and the storage of genetic information in DNA and RNA. 4. Genomes of viruses, prokaryotic, and eukaryotic organisms: organization and key differences. 5. DNA replication: fundamental principles and biological significance. 6. Gene expression I: transcription and post-transcriptional RNA modifications. 7. Gene expression II: translation and post-translational protein modifications. 8. Regulation of gene expression. Fundamental regulatory mechanisms. 9. Changes in genetic information: mutations, recombination, and mobile genetic elements. 10. Horizontal gene transfer: conjugation, transformation, and transduction. 11. Fundamentals of molecular biology techniques: sampling, nucleic acid isolation, and electrophoresis. 12. Amplification and sequencing techniques: PCR and its modification, DNA sequencing. 13. Fundamentals of genetic engineering: DNA cleavage and ligation, CRISPR, and gene cloning. 14. Practical applications of molecular biology. Genomics and genetically modified organisms.
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Learning activities and teaching methods
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Lecturing, Dialogic (Discussion, conversation, brainstorming), Simple experiments
- Participation in classes
- 56 hours per semester
- Home preparation for classes
- 24 hours per semester
- Preparation for course credit
- 10 hours per semester
- Preparation for examination
- 60 hours per semester
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| prerequisite |
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| Knowledge |
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| Knowledge of basic biology, general microbiology and biochemistry. |
| Knowledge of basic biology, general microbiology and biochemistry. |
| Skills |
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| Basic operations in the microbiology laboratory, principles of aseptic work. |
| Basic operations in the microbiology laboratory, principles of aseptic work. |
| learning outcomes |
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| Knowledge |
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| about morphological cell composition |
| about morphological cell composition |
| about structure of basic biomacromolecules - nucleic acids and proteins |
| about structure of basic biomacromolecules - nucleic acids and proteins |
| about the expression of genetic information |
| about the expression of genetic information |
| about the mechanisms of genetic information change (mutation, conjugation, transformation, transduction) |
| about the mechanisms of genetic information change (mutation, conjugation, transformation, transduction) |
| about basic methods used in molecular biology |
| about basic methods used in molecular biology |
| Skills |
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| apply various methods of DNA isolation, interpret and evaluate the results and determine the quality, purity and concentration of the DNA obtained |
| apply various methods of DNA isolation, interpret and evaluate the results and determine the quality, purity and concentration of the DNA obtained |
| perform and interpret the results obtained by agarose gel electrophoresis |
| perform and interpret the results obtained by agarose gel electrophoresis |
| design and carry out DNA amplification by PCR |
| design and carry out DNA amplification by PCR |
| use appropriate restriction enzymes depending on the intended DNA manipulation, apply restriction analysis and evaluate the results |
| use appropriate restriction enzymes depending on the intended DNA manipulation, apply restriction analysis and evaluate the results |
| analyse DNA sequencing results to identify microorganisms |
| analyse DNA sequencing results to identify microorganisms |
| teaching methods |
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| Knowledge |
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| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Lecturing |
| Lecturing |
| Educational trip |
| Educational trip |
| Skills |
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| Simple experiments |
| Simple experiments |
| Educational trip |
| Educational trip |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Analysis of works made by the student (Technical products) |
| Analysis of works made by the student (Technical products) |
| Written examination |
| Written examination |
| Grade (Using a grade system) |
| Grade (Using a grade system) |
| Didactic test |
| Didactic test |
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Recommended literature
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ALBERTS, B. Základy buněčné biologie. Ústí nad Labem: Espero, 2001. ISBN 80-902906-2-0.
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BROWN, T.A. Gene Cloning and DNA Analysis: An Introduction. 7th Ed.. Chichester: Wiley Blackwell, 2016. ISBN 978-1-119-07256-0.
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CRAIG, N.L. Molecular Biology: Principles of Genome Function. 2nd Ed.. Oxford University Press. ISBN 978-0-19-965857-2.
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ROSYPAL, S. úvod do molekulární biologie. Brno: Stanislav Rosypal, 2006. ISBN 80-092562-5-2.
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SNUSTAD, D.P., SIMMONS, M.J., RELICHOVÁ, J. Genetika. Brno: MU Brno, 2005. ISBN 978-80-210-4852-2.
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ŠMARDA, J. Metody molekulární biologie. Brno: MU Brno, 2005. ISBN 80-210-3841-1.
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