Course: Fundamentals of recombinant technologies

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Course title Fundamentals of recombinant technologies
Course code TUIOZP/TP6ZR
Organizational form of instruction Lecture + Seminary
Level of course Bachelor
Year of study not specified
Semester Summer
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Janalíková Magda, doc. Mgr. Ph.D.
  • Koutný Marek, prof. Mgr. Ph.D.
  • Baťová Adéla, Mgr.
Course content
- Basic concepts, definitions, importance and perspectives. - Methods of DNA isolation, purification and manipulation. - DNA cloning, amplification techniques, their applications. - Molecular analysis of the genome. Gene libraries. - Mutagenesis - possibilities, variants, significance. - General characteristics of vectors for gene transfer to prokaryotes and eukaryotes, expression systems. - Gene cloning, gene engineering. - Genetically modified organisms, transgenesis - definition, legislation, use. - Gene therapies. - Application of recombinant DNA methods in food, agriculture, biotechnology, industry and healthcare.

Learning activities and teaching methods
Monologic (Exposition, lecture, briefing), Dialogic (Discussion, conversation, brainstorming), Practice exercises
  • Preparation for examination - 120 hours per semester
learning outcomes
Knowledge
describe the fundamental concepts in the field of recombinant technologies
describe the fundamental concepts in the field of recombinant technologies
explain mutagenesis, genome analysis, and the principle of gene libraries
explain mutagenesis, genome analysis, and the principle of gene libraries
define genetically modified organisms and the principle of cloning
define genetically modified organisms and the principle of cloning
clarify the use of vectors in transgenesis in prokaryotes and eukaryotes
clarify the use of vectors in transgenesis in prokaryotes and eukaryotes
provide examples of the application of recombinant DNA methods in food industry, agriculture, biotechnology, and healthcare
provide examples of the application of recombinant DNA methods in food industry, agriculture, biotechnology, and healthcare
Skills
find a scientific article on recombinant technologies using scientific databases
find a scientific article on recombinant technologies using scientific databases
search for a method of DNA manipulation in the scientific article
search for a method of DNA manipulation in the scientific article
explain a specific method used within the scope of the scientific study
explain a specific method used within the scope of the scientific study
distinguish amplification techniques and recombinant DNA techniques
distinguish amplification techniques and recombinant DNA techniques
justify the use of recombinant DNA techniques within a specific scientific study
justify the use of recombinant DNA techniques within a specific scientific study
teaching methods
Knowledge
Monologic (Exposition, lecture, briefing)
Monologic (Exposition, lecture, briefing)
Methods for working with texts (Textbook, book)
Methods for working with texts (Textbook, book)
Skills
Dialogic (Discussion, conversation, brainstorming)
Dialogic (Discussion, conversation, brainstorming)
Practice exercises
Practice exercises
Individual work of students
Individual work of students
assessment methods
Knowledge
Didactic test
Oral examination
Analysis of seminar paper
Written examination
Written examination
Didactic test
Analysis of seminar paper
Oral examination
Recommended literature
  • ALBERTS, B. Základy buněčné biologie. Ústí nad Labem: Espero, 2001. ISBN 80-902906-2-0.
  • Antao, Tiago. Bioinformatics with Python cookbook. Birmingham, UK. 2022.
  • BROWN, T.A. Gene Cloning and DNA Analysis: An Introduction. 7th Ed.. Chichester: Wiley Blackwell, 2016. ISBN 978-1-119-07256-0.
  • CRAIG, N.L. Molecular Biology: Principles of Genome Function. 2nd ed.. Oxford: Oxford University Press. ISBN 978-0-19-965857-2.
  • DALE, J., SCHANTZ, M., PLANT, N. From genes to genomes: concepts and applications of DNA technology, 3rd ed.. Chichester, West Sussex: Wiley-Blackwell, 2012. ISBN 978-0-470-68385-9.
  • ROSYPAL, S., DOŠKAŘ, J. Úvod do molekulární biologie. 3. díl., 2. rozš. vyd.. Brno: Stanislav Rosypal, 1997.
  • RUML, T., RUMLOVÁ, M., PAČES, V. Genové inženýrství. VŠCHT, Praha, 2012.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester