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Lecturer(s)
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Janalíková Magda, doc. Mgr. Ph.D.
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Koutný Marek, prof. Mgr. Ph.D.
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Baťová Adéla, Mgr.
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Course content
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- Basic concepts, definitions, importance and perspectives. - Methods of DNA isolation, purification and manipulation. - DNA cloning, amplification techniques, their applications. - Molecular analysis of the genome. Gene libraries. - Mutagenesis - possibilities, variants, significance. - General characteristics of vectors for gene transfer to prokaryotes and eukaryotes, expression systems. - Gene cloning, gene engineering. - Genetically modified organisms, transgenesis - definition, legislation, use. - Gene therapies. - Application of recombinant DNA methods in food, agriculture, biotechnology, industry and healthcare.
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Learning activities and teaching methods
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Monologic (Exposition, lecture, briefing), Dialogic (Discussion, conversation, brainstorming), Practice exercises
- Preparation for examination
- 120 hours per semester
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| learning outcomes |
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| Knowledge |
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| describe the fundamental concepts in the field of recombinant technologies |
| describe the fundamental concepts in the field of recombinant technologies |
| explain mutagenesis, genome analysis, and the principle of gene libraries |
| explain mutagenesis, genome analysis, and the principle of gene libraries |
| define genetically modified organisms and the principle of cloning |
| define genetically modified organisms and the principle of cloning |
| clarify the use of vectors in transgenesis in prokaryotes and eukaryotes |
| clarify the use of vectors in transgenesis in prokaryotes and eukaryotes |
| provide examples of the application of recombinant DNA methods in food industry, agriculture, biotechnology, and healthcare |
| provide examples of the application of recombinant DNA methods in food industry, agriculture, biotechnology, and healthcare |
| Skills |
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| find a scientific article on recombinant technologies using scientific databases |
| find a scientific article on recombinant technologies using scientific databases |
| search for a method of DNA manipulation in the scientific article |
| search for a method of DNA manipulation in the scientific article |
| explain a specific method used within the scope of the scientific study |
| explain a specific method used within the scope of the scientific study |
| distinguish amplification techniques and recombinant DNA techniques |
| distinguish amplification techniques and recombinant DNA techniques |
| justify the use of recombinant DNA techniques within a specific scientific study |
| justify the use of recombinant DNA techniques within a specific scientific study |
| teaching methods |
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| Knowledge |
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| Monologic (Exposition, lecture, briefing) |
| Monologic (Exposition, lecture, briefing) |
| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| Skills |
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| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Practice exercises |
| Practice exercises |
| Individual work of students |
| Individual work of students |
| assessment methods |
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| Knowledge |
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| Didactic test |
| Oral examination |
| Analysis of seminar paper |
| Written examination |
| Written examination |
| Didactic test |
| Analysis of seminar paper |
| Oral examination |
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Recommended literature
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ALBERTS, B. Základy buněčné biologie. Ústí nad Labem: Espero, 2001. ISBN 80-902906-2-0.
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Antao, Tiago. Bioinformatics with Python cookbook. Birmingham, UK. 2022.
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BROWN, T.A. Gene Cloning and DNA Analysis: An Introduction. 7th Ed.. Chichester: Wiley Blackwell, 2016. ISBN 978-1-119-07256-0.
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CRAIG, N.L. Molecular Biology: Principles of Genome Function. 2nd ed.. Oxford: Oxford University Press. ISBN 978-0-19-965857-2.
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DALE, J., SCHANTZ, M., PLANT, N. From genes to genomes: concepts and applications of DNA technology, 3rd ed.. Chichester, West Sussex: Wiley-Blackwell, 2012. ISBN 978-0-470-68385-9.
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ROSYPAL, S., DOŠKAŘ, J. Úvod do molekulární biologie. 3. díl., 2. rozš. vyd.. Brno: Stanislav Rosypal, 1997.
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RUML, T., RUMLOVÁ, M., PAČES, V. Genové inženýrství. VŠCHT, Praha, 2012.
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