|
Lecturer(s)
|
-
Koutný Marek, prof. Mgr. Ph.D.
-
Pleva Pavel, Ing. Ph.D.
-
Jančová Petra, Mgr. Ph.D.
-
Baťová Adéla, Mgr.
-
Sotolářová Jitka, Ing.
-
Šenkárová Lenka, Ing. Ph.D.
-
Bábková Dagmar, Ing.
|
|
Course content
|
1. Introductory lesson: Laboratory work safety training, weighing, pipetting, measuring with UV / VIS spectrophotometer, buffer preparation. 2. Carbohydrates: The chemical properties of carbohydrates, monosaccharides group reactions, identification of the unknown sample, hydrolysis of polysaccharide. 3. Amino acids, proteins: Reactions of amino acid side chains, the identification of the unknown sample, the denaturation of proteins. 4. Afinity chromatography. 5. Enzymes, enzyme activity: Monitoring and quantification amylase activity of human saliva, proof of peroxidase activity in milk. 6. Purification of an enzyme: Two-step purification of phenoloxidases from fruiting bodies of mushrooms, acetone fractionation, ion exchange chromatography, activity detection. 7. Lipids: Isolation of phospholipids from egg yolk; evidence reactions (cholesterol, choline, phosphoric acid); emulsifying ability of phosphatides.
|
|
Learning activities and teaching methods
|
Simple experiments
- Participation in classes
- 28 hours per semester
- Home preparation for classes
- 22 hours per semester
- Preparation for course credit
- 10 hours per semester
|
| prerequisite |
|---|
| Knowledge |
|---|
| Knowledge of organic chemistry and biochemistry. |
| Knowledge of organic chemistry and biochemistry. |
| learning outcomes |
|---|
| Knows the basic concepts used in the biochemical laboratory. |
| Knows the basic concepts used in the biochemical laboratory. |
| He has the theoretical basis of basic operations used in the biochemical laboratory. |
| He has the theoretical basis of basic operations used in the biochemical laboratory. |
| Skills |
|---|
| have practical experience with basic operations in a biochemical laboratory. |
| have practical experience with basic operations in a biochemical laboratory. |
| know to prepare buffers and work in a buffered environment. |
| know to prepare buffers and work in a buffered environment. |
| know the basics of working with proteins and enzymes. |
| know the basics of working with proteins and enzymes. |
| know the basics of protein purification. |
| know the basics of protein purification. |
| teaching methods |
|---|
| Knowledge |
|---|
| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Skills |
|---|
| Simple experiments |
| Simple experiments |
| Practice exercises |
| Practice exercises |
| assessment methods |
|---|
| Knowledge |
|---|
| Analysis of another type of paper written by the student (Casuistry, diary, plan ...) |
| Analysis of another type of paper written by the student (Casuistry, diary, plan ...) |
| Grade (Using a grade system) |
| Grade (Using a grade system) |
|
Recommended literature
|
-
KODÍČEK M., VALENTOVÁ O., HYNEK R. Biochemie; chemický pohled na biologický svět. Praha: VŠCHT v Praze, 2015. ISBN 978-80-7080-927-3.
-
Robert K. Murray. Harperova ilustrovaná biochemie. Galén, spol. s r.o., 2012. ISBN 978-80-7262-907-7.
|