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Lecturer(s)
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Buňková Leona, prof. RNDr. Ph.D.
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Huňová Ivana, Ing.
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Píšová Pavlína, Ing.
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Course content
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1. Light microscopy - principle, methods of observing microorganisms, preparation of slides. 2. Fluorescence and electron microscopy - principle, methods of observing microorganisms. 3. Bacterial colonies - properties, characteristics, growth. 4. Colonies of microscopic fungi (yeasts and moulds) - properties, characteristic features, growth. 5. Cell structure of bacteria I - cytoplasmic membrane, cytoplasm, chromosome, plasmids, ribosomes. 6. Bacterial cell structure II - cell wall, flagellum. 7. Bacterial cell structure III - surface structures, inclusions, bacteria with less typical cell structure. 8. Structure of the archaeal cell and its differences from the bacterial cell. 9. Cell structure of microscopic fungi I - cell wall, cytoplasmic membrane, nucleus. 10. Cell structure of microscopic fungi II - membrane organelles and other cytoplasmic structures. Differences in the structure of yeasts and microscopic filamentous fungi. 11. Reproduction of prokaryotic and eukaryotic microorganisms. 12. Microbial biofilm. 13. Independent work I - methods of monitoring microorganisms. 14. Independent work II - methods of monitoring microorganisms.
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Learning activities and teaching methods
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- Participation in classes
- 56 hours per semester
- Home preparation for classes
- 40 hours per semester
- Preparation for course credit
- 24 hours per semester
- Preparation for examination
- 30 hours per semester
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| prerequisite |
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| Knowledge |
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| Knowledge of basic biology and general microbiology. |
| Knowledge of basic biology and general microbiology. |
| Skills |
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| It builds on the basic skills acquired in the general microbiology laboratory, where the student can practically apply the basics of microscopy, aseptic work, inoculation and cultivation. |
| It builds on the basic skills acquired in the general microbiology laboratory, where the student can practically apply the basics of microscopy, aseptic work, inoculation and cultivation. |
| learning outcomes |
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| Knowledge |
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| Morphology of prokaryotic cells - bacteria and their function |
| Morphology of prokaryotic cells - bacteria and their function |
| Morphology of prokaryotic cells - archaea and their function |
| Morphology of prokaryotic cells - archaea and their function |
| Yeast structure and their importance |
| Yeast structure and their importance |
| The structure of moulds (microscopic filamentous fungi) and their importance |
| The structure of moulds (microscopic filamentous fungi) and their importance |
| Optical (light) microscopy and its applications |
| Optical (light) microscopy and its applications |
| Skills |
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| Student is able to use culture techniques for the isolation and identification of bacteria |
| Student is able to use culture techniques for the isolation and identification of bacteria |
| Student knows the possibilities of imaging microorganisms using different microscopic methods and staining |
| Student knows the possibilities of imaging microorganisms using different microscopic methods and staining |
| Student is able to recognize basic macro and/or microscopic features of prokaryotic microorganisms |
| Student is able to recognize basic macro and/or microscopic features of prokaryotic microorganisms |
| Student is able to recognize the basic macro/or microscopic features of eukaryotic microorganisms |
| Student is able to recognize the basic macro/or microscopic features of eukaryotic microorganisms |
| Student is able to distinguish the differences between prokaryotic and eukaryotic cells |
| Student is able to distinguish the differences between prokaryotic and eukaryotic cells |
| teaching methods |
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| Knowledge |
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| Lecturing |
| Lecturing |
| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| Skills |
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| Simple experiments |
| Simple experiments |
| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Individual work of students |
| Individual work of students |
| assessment methods |
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| Knowledge |
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| Grade (Using a grade system) |
| Grade (Using a grade system) |
| Written examination |
| Written examination |
| Analysis of works made by the student (Technical products) |
| Analysis of works made by the student (Technical products) |
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Recommended literature
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ALBERTS, B. Základy buněčné biologie. Ústí nad Labem: Espero, 2001. ISBN 80-902906-2-0.
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DEACON, J. Fungal biology. Chichester: Wiley Blackwell, 2006. ISBN 978-1-405-13066-0.
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KLABAN, V. Ekologie mikroorganismů: ilustrovaný lexikon biologie, ekologie a patogenity mikroorganismů. Praha: Galén, 2011. ISBN 978-80-7262-770-7.
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KOPECKÁ, J., ROTKOVÁ, G. . Skripta ke cvičení z obecné mikrobiologie, cytologie a morfologie bakterií. Brno: MU Brno,Elportál, 2017. ISBN 978-80-210-8787-3.
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MADIGAN, M. a kol. Brock biology of microorganisms. Boston: Pearson, 2015. ISBN 978-1-292-01831-7.
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MIESLEROVÁ, B., SEDLÁŘOVÁ, M., LEBEDA, A. Houby a houbám podobné organismy v biotechnologiích. Olomouc, 2016. ISBN 978-80-244-4983-8.
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NĚMEC, M., MATOULKOVÁ, D. Základy obecné mikrobiologie. Brno, 2015. ISBN 978-80-210-7923-6.
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