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Lecturer(s)
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Purevdorj Khatantuul, Ing. Ph.D.
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Riemel Jakub, Ing.
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Course content
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1. Introduction, work safety, laboratory instruments. 2. Laboratory instruments, basic laboratory calculations. 3. Weighing using scales and analytical scales. 4. Samples dissolving, volumetric flasks. 5. Pipetting. Dispersers. 6. Preparation of basic culture media. 7. Work in a laminary and biohazard box. 8. Light microscope - construction and properties. 9. Miscroscopy - permanent and native slides. 10. Miscroscopy - stained slides. 11. Microscopy - inversion lenses. 12. Basic microbiology techniques. 13. Basic techniques in molecular biology. 14. Exam.
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Learning activities and teaching methods
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- Participation in classes
- 42 hours per semester
- Home preparation for classes
- 25 hours per semester
- Preparation for course credit
- 23 hours per semester
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| learning outcomes |
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| Knowledge |
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| principles of aseptic technique in the microbiological laboratory |
| principles of aseptic technique in the microbiological laboratory |
| description of the basic aids and tools used in the microbiology and molecular biology laboratory |
| description of the basic aids and tools used in the microbiology and molecular biology laboratory |
| description of types of nutrient media used for inoculation of microorganisms |
| description of types of nutrient media used for inoculation of microorganisms |
| name the parts of the optical microscope |
| name the parts of the optical microscope |
| name and describe the basic methods of staining microorganisms and cellular components |
| name and describe the basic methods of staining microorganisms and cellular components |
| Skills |
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| ability to operate and use the basic aids and tools used in the microbiology and molecular biology laboratory |
| ability to operate and use the basic aids and tools used in the microbiology and molecular biology laboratory |
| ability to prepare culture media for the cultivation of microorganisms |
| ability to prepare culture media for the cultivation of microorganisms |
| ability to prepare a fixed specimens for light microscopy |
| ability to prepare a fixed specimens for light microscopy |
| ability to examine a specimen with a light microscope |
| ability to examine a specimen with a light microscope |
| ability to inoculate microorganisms using basic inoculation techniques |
| ability to inoculate microorganisms using basic inoculation techniques |
| teaching methods |
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| Knowledge |
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| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| E-learning |
| E-learning |
| Skills |
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| Simple experiments |
| Simple experiments |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Analysis of another type of paper written by the student (Casuistry, diary, plan ...) |
| Analysis of another type of paper written by the student (Casuistry, diary, plan ...) |
| Preparation of a presentation, giving a presentation |
| Preparation of a presentation, giving a presentation |
| Systematic observation of the student |
| Systematic observation of the student |
| Written examination |
| Written examination |
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Recommended literature
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BÁLEŠ, V. a kol. Biochemické technológie: Biochemical Technologies. Bratislava: AB-ART, 2003. ISBN 80-89006-75-2.
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KOTRBA, P., BABŮREK, I., KREJZLÍK, Z. Návody ke cvičením z biologie. Praha: VŠCHT Praha, 2006. ISBN 80-7080-623-0.
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POLLACK, Robert A. Laboratory exercises in microbiology. 3rd ed.. Hoboken, NJ: John Wiley, 2009. ISBN 978-0-470-13392-7.
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SEIDMAN, L. Basic Laboratory Calculations for Biotechnology. San Francisco, Pearson, 2008. ISBN 978-0-13-223810-6.
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SEIDMAN, L., MOORE, C. J. Basic Laboratory Methods for Biotechnology, 2nd ed.. San Francisco, Pearson, 2009. ISBN 978-0-321-57014-7.
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ZAJONCOVÁ, L., TARKOWSKI, P. Základní chemické výpočty pro biochemiky a biotechnology. Olomouc: UP Olomouc, 2016. ISBN 9788024450025.
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