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Lecturer(s)
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Pleva Pavel, Ing. Ph.D.
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Janalíková Magda, doc. Mgr. Ph.D.
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Šerá Jana, Ing. Ph.D.
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Buňková Leona, prof. RNDr. Ph.D.
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Course content
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1. Introduction to the study of molecular biology. 2. Living organisms and cell composition. 3. Nucleic acids - types, structure, properties. 4. Genome of viruses, prokaryotic and eukaryotic organisms. 5. DNA replication. 6. Transcription and translation. 7. Regulation of gene expression. 8. Change of genetic information - mutations, transposition, transformation, conjugation, transduction. 9. Molecular biological methods and their use I - isolation of nucleic acids and proteins, electrophoresis, hybridization. 10. Molecular biological methods and their use II - PCR, real-time PCR and their modifications. 11. Molecular biological methods and their use III - restriction cleavage of nucleic acids, RFLP, DGGE, study of proteins. 12. Genetic engineering and gene cloning I. 13. Genetic engineering and gene cloning II. 14. Genetically modified organisms and relation to the food industry.
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Learning activities and teaching methods
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Lecturing, Dialogic (Discussion, conversation, brainstorming), Simple experiments
- Participation in classes
- 16 hours per semester
- Home preparation for classes
- 44 hours per semester
- Preparation for course credit
- 26 hours per semester
- Preparation for examination
- 34 hours per semester
- Participation in classes
- 56 hours per semester
- Home preparation for classes
- 24 hours per semester
- Preparation for course credit
- 16 hours per semester
- Preparation for examination
- 24 hours per semester
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| prerequisite |
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| Knowledge |
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| Knowledge of basic biology, general microbiology and biochemistry. |
| Knowledge of basic biology, general microbiology and biochemistry. |
| Skills |
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| Basic operations in the microbiology laboratory, principles of aseptic work. |
| Basic operations in the microbiology laboratory, principles of aseptic work. |
| learning outcomes |
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| Knowledge |
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| about morphological cell composition |
| about morphological cell composition |
| about structure of basic biomacromolecules - nucleic acids and proteins |
| about structure of basic biomacromolecules - nucleic acids and proteins |
| about the expression of genetic information |
| about the expression of genetic information |
| about the mechanisms of genetic information change (mutation, conjugation, transformation, transduction) |
| about the mechanisms of genetic information change (mutation, conjugation, transformation, transduction) |
| about basic methods used in molecular biology |
| about basic methods used in molecular biology |
| Skills |
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| apply various methods of DNA isolation, interpret and evaluate the results and determine the quality, purity and concentration of the DNA obtained |
| apply various methods of DNA isolation, interpret and evaluate the results and determine the quality, purity and concentration of the DNA obtained |
| perform and interpret the results obtained by agarose gel electrophoresis |
| perform and interpret the results obtained by agarose gel electrophoresis |
| design and carry out DNA amplification by PCR |
| design and carry out DNA amplification by PCR |
| use appropriate restriction enzymes depending on the intended DNA manipulation, apply restriction analysis and evaluate the results |
| use appropriate restriction enzymes depending on the intended DNA manipulation, apply restriction analysis and evaluate the results |
| analyse DNA sequencing results to identify microorganisms |
| analyse DNA sequencing results to identify microorganisms |
| teaching methods |
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| Knowledge |
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| Dialogic (Discussion, conversation, brainstorming) |
| Lecturing |
| Lecturing |
| Dialogic (Discussion, conversation, brainstorming) |
| Skills |
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| Simple experiments |
| Simple experiments |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Analysis of works made by the student (Technical products) |
| Analysis of works made by the student (Technical products) |
| Written examination |
| Written examination |
| Grade (Using a grade system) |
| Grade (Using a grade system) |
| Didactic test |
| Didactic test |
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Recommended literature
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ALBERTS, B. Základy buněčné biologie. Ústí nad Labem: Espero, 2001. ISBN 80-902906-2-0.
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BROWN, T.A. Gene Cloning and DNA Analysis: An Introduction. 7th Ed.. Chichester: Wiley Blackwell, 2016. ISBN 978-1-119-07256-0.
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CRAIG, N.L. Molecular Biology: Principles of Genome Function. 2nd Ed.. Oxford University Press. ISBN 978-0-19-965857-2.
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ROSYPAL, S. úvod do molekulární biologie. Brno: Stanislav Rosypal, 2006. ISBN 80-092562-5-2.
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SNUSTAD, D.P., SIMMONS, M.J., RELICHOVÁ, J. Genetika. Brno: MU Brno, 2005. ISBN 978-80-210-4852-2.
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ŠMARDA, J. Metody molekulární biologie. Brno: MU Brno, 2005. ISBN 80-210-3841-1.
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