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Lecturer(s)
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Buňková Leona, prof. RNDr. Ph.D.
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Purevdorj Khatantuul, Ing. Ph.D.
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Course content
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- Introduction to the study - definition of biotechnology, microbial biotechnology, requirements for industrial microorganisms. - Prokaryotic microorganisms used in biotechnology. - Eukaryotic microorganisms used in biotechnology. - Cell cycle. Growth curve of microorganisms. Growth constants. - Factors affecting the growth and reproduction of microorganisms. Biomass production. - Overview of the main pathways of primary and secondary metabolism of microorganisms and their importance in biotechnology. - Cultivation and production media - requirements, raw materials, composition, sterilization. - Methods of cultivation of microorganisms in a liquid medium - batch, flow, semi-continuous and continuous cultivation. - Cultivation on solid substrate. Biofilm in biotechnology. - Specific features of the cultivation of phototrophic organisms (cyanobacteria, algae and plant cells) and animal cells. - Bioreactors - division, construction, use. - Biotechnological production of primary metabolites. - Biotechnological production of secondary metabolites. - Biotransformation - basic characteristics of processes, examples.
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Learning activities and teaching methods
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Monologic (Exposition, lecture, briefing), Dialogic (Discussion, conversation, brainstorming), Simple experiments
- Preparation for examination
- 90 hours per semester
- Preparation for examination
- 90 hours per semester
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| learning outcomes |
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| Knowledge |
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| list and describe the basic strategies for the cultivation of organisms used in biotechnology |
| list and describe the basic strategies for the cultivation of organisms used in biotechnology |
| describe substrates for the cultivation of organisms used in biotechnology |
| describe substrates for the cultivation of organisms used in biotechnology |
| describe bioreactors used for biotechnological applications |
| describe bioreactors used for biotechnological applications |
| explain the differences between static and continuous cultivation of (micro)organisms |
| explain the differences between static and continuous cultivation of (micro)organisms |
| list the most important groups of organisms used in biotechnology |
| list the most important groups of organisms used in biotechnology |
| Skills |
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| design culture media for the cultivation of microorganisms used in biotechnology |
| design culture media for the cultivation of microorganisms used in biotechnology |
| optimize the cultivation conditions of 5 (micro)organisms most often used in biotechnology |
| optimize the cultivation conditions of 5 (micro)organisms most often used in biotechnology |
| implement the determination of the growth curve of bacteria |
| implement the determination of the growth curve of bacteria |
| design organisms that can be used for the biotechnological production of additives used in the food industry |
| design organisms that can be used for the biotechnological production of additives used in the food industry |
| design organisms usable for the biotechnological production of substances used in pharmacy, medicine or environmental protection |
| design organisms usable for the biotechnological production of substances used in pharmacy, medicine or environmental protection |
| teaching methods |
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| Knowledge |
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| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Monologic (Exposition, lecture, briefing) |
| Monologic (Exposition, lecture, briefing) |
| Skills |
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| Simple experiments |
| Simple experiments |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Analysis of works made by the student (Technical products) |
| Analysis of works made by the student (Technical products) |
| Grade (Using a grade system) |
| Grade (Using a grade system) |
| Didactic test |
| Didactic test |
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Recommended literature
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HARZEVILI, F.D., CHEN, H. Microbial Biotechnology: Progress and Trends. Boca Raton: CRC Press, 2015. ISBN 978-1-4822-4520-2.
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KADLEC, P., MELZOCH, K., VOLDŘICH, M. Procesy a zařízení v potravinářství a biotechnologiích. Ostrava: KEY Publishing, 2013. ISBN 978-80-7418-163-4.
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KAPRÁLEK, F. Základy bakteriologie. Praha : Karolinum, 1999. ISBN 80-7184-811-5.
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LEE, Y.K. Microbial Biotechnology: Principles and Applications, 3rd ed.. Singapore: World Scientific, 2013. ISBN 978-981-4366-82-3.
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MIESLEROVÁ, B., SEDLÁŘOVÁ, M., LEBEDA, A. Houby a houbám podobné organismy v biotechnologiích. Olomouc, 2016. ISBN 978-80-244-4983-8.
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