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Lecturer(s)
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Jančová Petra, Mgr. Ph.D.
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Buňková Leona, prof. RNDr. Ph.D.
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Šerá Jana, Ing. Ph.D.
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Course content
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1. Definition of bioanalytical methods. Sampling. Principles of working with biological material. Classification of methods. 2. Homogenization of tissues and extraction of cell contents. Dialysis, lyophilization, filtration and ultrafiltration. 3. Nucleic acid analysis I - nucleic acid isolation, polymerase chain reaction. 4. Nucleic acid analysis II - hybridization, DNA fingerprinting, sequencing. 5. Imaging methods. 6. Conventional culture microbiological methods. 7. Modern methods used in the analysis of microorganisms. 8. Immunochemical methods. 9. Bioanalytical methods using radionuclides. 10. Spectroscopic methods. 11. Chromatographic methods. 12. Electromigration methods. 13. Methods for the study of proteins. Enzymatic analytical methods. 14. Electrochemical methods. Biosensors and biochips.
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Learning activities and teaching methods
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Lecturing, Dialogic (Discussion, conversation, brainstorming), Methods for working with texts (Textbook, book), Simple experiments, Teamwork, Individual work of students
- Participation in classes
- 16 hours per semester
- Home preparation for classes
- 50 hours per semester
- Preparation for course credit
- 24 hours per semester
- Preparation for examination
- 30 hours per semester
- Participation in classes
- 56 hours per semester
- Home preparation for classes
- 30 hours per semester
- Preparation for course credit
- 14 hours per semester
- Preparation for examination
- 20 hours per semester
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| prerequisite |
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| Knowledge |
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| Knowledge of basic biology, general microbiology and biochemistry. |
| Knowledge of basic biology, general microbiology and biochemistry. |
| learning outcomes |
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| define the basics of working with biological material |
| define the basics of working with biological material |
| describe the steps in the analysis of biological samples |
| describe the steps in the analysis of biological samples |
| list and characterise sample preparation methods |
| list and characterise sample preparation methods |
| describe optical and bioanalytical separation methods (filtration, chromatographic, electrochemical, electromigration) |
| describe optical and bioanalytical separation methods (filtration, chromatographic, electrochemical, electromigration) |
| characterize biochemical, immunochemical, microbiological and molecular biological methods |
| characterize biochemical, immunochemical, microbiological and molecular biological methods |
| Skills |
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| apply the principles of working with biological material |
| apply the principles of working with biological material |
| propose appropriate methods for the analysis of a given sample |
| propose appropriate methods for the analysis of a given sample |
| perform common methods of isolation of selected sample components for their subsequent determination |
| perform common methods of isolation of selected sample components for their subsequent determination |
| operate laboratory apparatus under the supervision of the teacher |
| operate laboratory apparatus under the supervision of the teacher |
| practical knowledge of modern bioanalytical (especially molecular biological) methods (e.g. isolation and purification of DNA using kits, polymerase chain reaction) |
| practical knowledge of modern bioanalytical (especially molecular biological) methods (e.g. isolation and purification of DNA using kits, polymerase chain reaction) |
| teaching methods |
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| Knowledge |
|---|
| Lecturing |
| Lecturing |
| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Individual work of students |
| Individual work of students |
| Skills |
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| Simple experiments |
| Simple experiments |
| Teamwork |
| Teamwork |
| Demonstration |
| Demonstration |
| Individual work of students |
| Individual work of students |
| assessment methods |
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| Knowledge |
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| Grade (Using a grade system) |
| Written examination |
| Written examination |
| Grade (Using a grade system) |
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Recommended literature
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ELLIOTT, W.H. Biochemistry and molecular biology. Oxford: Oxford University Press, 2005. ISBN 199271992.
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KODÍČEK, M. Biofysikální chemie. Praha: Academia, 2013. ISBN 978-80-200-2241-7.
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Králová B. a kol. Bioanalytické metody, 3. vydání. VŠCHT Praha. 2014.
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MANZ, A. Bioanalytical chemistry. London: Imperial College Press, 2015. ISBN 978-1-78326-671-5.
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ZÁRUBA, K. Analytická chemie. Praha: VŠCHT Praha, 2016. ISBN 978-80-7080-950-1.
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