Lecturer(s)
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Koutný Marek, prof. Mgr. Ph.D.
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Course content
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1. Work with proteins and enzymes, obtaining and preparation of proteins. 2. Protein purification, storage, activity determination, protein determination. 3. Basics of enzyme kinetics. 4. Events on biological membranes, transport, compartmentation of biochemical processes. 5. Transmission of nerve excitation, signaling substances, transmission of information across the membrane, biochemistry of vision, biochemistry of muscle contraction. 6. Biochemistry of blood, transport of substances through blood, clotting. 7. Immune system, antibodies, preparation and use of antibodies. 8. Xenobiochemistry. Free radicals, reactive oxygen species, oncogenesis. 9. Photosynthesis, light phase. 10. Photosynthesis, dark phase. 11. Alternative respiration. 12. Secondary metabolites I. 13. Secondary metabolites II. 14. New trends, discoveries and technologies in the field of biochemistry and related fields.
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Learning activities and teaching methods
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Lecturing, Monologic (Exposition, lecture, briefing), Demonstration, Activating (Simulation, games, dramatization), Practice exercises, Individual work of students
- Participation in classes
- 8 hours per semester
- Home preparation for classes
- 42 hours per semester
- Preparation for course credit
- 35 hours per semester
- Preparation for examination
- 35 hours per semester
- Participation in classes
- 56 hours per semester
- Preparation for course credit
- 34 hours per semester
- Preparation for examination
- 30 hours per semester
- Home preparation for classes
- 32 hours per semester
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prerequisite |
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Knowledge |
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Knowledge of basics of biochemistry. |
Knowledge of basics of biochemistry. |
Knowledge of organic chemistry. |
Knowledge of organic chemistry. |
learning outcomes |
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knows structures and properties of basic biomolecules. |
knows structures and properties of basic biomolecules. |
knows the principles of function of biomolecules and their relationship to structure. |
knows the principles of function of biomolecules and their relationship to structure. |
knows processes and principles of the central dogma of biomolecule synthesis. |
knows processes and principles of the central dogma of biomolecule synthesis. |
knows groups of secondary metabolites and their examples. |
knows groups of secondary metabolites and their examples. |
knows functioning of photosynthesis. |
knows functioning of photosynthesis. |
knows principles of work with proteins. |
knows principles of work with proteins. |
knows the functioning of muscle contraction, signal transduction, vision. |
knows the functioning of muscle contraction, signal transduction, vision. |
knows principles of the processes of ontogenesis, oncogenesis, ROS, ... |
knows principles of the processes of ontogenesis, oncogenesis, ROS, ... |
Skills |
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knows to choose a suitable buffer for working with proteins. |
knows to choose a suitable buffer for working with proteins. |
prepare a suitable buffer. |
prepare a suitable buffer. |
characterize the secondary metabolite and find information on its properties. |
characterize the secondary metabolite and find information on its properties. |
teaching methods |
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Knowledge |
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Monologic (Exposition, lecture, briefing) |
Demonstration |
Demonstration |
Lecturing |
Lecturing |
Monologic (Exposition, lecture, briefing) |
Methods for working with texts (Textbook, book) |
Methods for working with texts (Textbook, book) |
Skills |
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Activating (Simulation, games, dramatization) |
Activating (Simulation, games, dramatization) |
Dialogic (Discussion, conversation, brainstorming) |
Dialogic (Discussion, conversation, brainstorming) |
Individual work of students |
Individual work of students |
Practice exercises |
Practice exercises |
assessment methods |
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Knowledge |
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Oral examination |
Written examination |
Didactic test |
Didactic test |
Oral examination |
Grade (Using a grade system) |
Grade (Using a grade system) |
Written examination |
Recommended literature
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Kodíček, Milan. Biochemické pojmy : výkladový slovník. Vyd. 1. Praha : Vysoká škola chemicko-technologická, 2004. ISBN 807080551X.
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KODÍČEK,M., VALENTOVÁ,O., HYNEK,R. Biochemie: chemický pohled na biologický svět. Praha: VŠCHTv Praze, 2015. ISBN 978-80-7080-927-3.
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LEHNINGER A., NELSON D.L., YOUNG P. Principles of Biochemistry. W.H. Freeman & Company, 2007.
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LEHNINGER,A., NELSON,D.L., YOUNG,P. Principles of Biochemistry. W.H. Freeman & Company, 2007. ISBN 0-7167-3051-0.
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MURRAY, R.K., GRANNER, D. K., MAYES, P.A., RODWELL V.W. Harperova biochemie. Jinočany, 2002.
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MURRAY,R.K. Harperova ilustrovaná biochemie,5. české vyd., 1. v nakl. Galén. Praha: Galén, 2012. ISBN 978-80-7262-907-7.
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Nelson, David L. Lehninger principles of biochemistry. 5th ed. New York : W.H. Freeman, 2008. ISBN 978-0-7167-7108-1.
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Šípal a kol. Biochemie. SPN Praha, 1992. ISBN 8004217362.
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ŠÍPAL, Z. a kol. Biochemie. Praha : SPN, 1992. ISBN 80-04-21736-2.
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