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Lecturer(s)
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Purevdorj Khatantuul, Ing. Ph.D.
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Course content
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1. Introductory lesson, work safety, acquaintance with laboratory aids. 2. Basic laboratory calculations. 3. Weighing on pre-weights and analytical balances. 4. Dissolution of samples, measuring vessels. 5. Filtration, yield. 6. Working with pipettes and dispensers. 7. Work with burettes, titration. 8. Light microscope - construction and properties. 9. Microscopy - permanent and native slides. 10. Microscopy - stained slides. 11. Microscopy - use of immersion objective. 12. Basic microbiological techniques. 13. Work in laminar and biohazard box. 14. Basic techniques of molecular biology.
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Learning activities and teaching methods
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- Participation in classes
- 12 hours per semester
- Home preparation for classes
- 45 hours per semester
- Preparation for course credit
- 33 hours per semester
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| learning outcomes |
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| Knowledge |
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| principles of aseptic technique in the microbiological laboratory |
| principles of aseptic technique in the microbiological laboratory |
| description of the basic aids and tools used in the microbiology and molecular biology laboratory |
| description of the basic aids and tools used in the microbiology and molecular biology laboratory |
| description of types of nutrient media used for inoculation of microorganisms |
| description of types of nutrient media used for inoculation of microorganisms |
| name the parts of the optical microscope |
| name the parts of the optical microscope |
| name and describe the basic methods of staining microorganisms and cellular components |
| name and describe the basic methods of staining microorganisms and cellular components |
| Skills |
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| ability to operate and use the basic aids and tools used in the microbiology and molecular biology laboratory |
| ability to operate and use the basic aids and tools used in the microbiology and molecular biology laboratory |
| ability to prepare culture media for the cultivation of microorganisms |
| ability to prepare culture media for the cultivation of microorganisms |
| ability to prepare a fixed specimens for light microscopy |
| ability to prepare a fixed specimens for light microscopy |
| ability to examine a specimen with a light microscope |
| ability to examine a specimen with a light microscope |
| ability to inoculate microorganisms using basic inoculation techniques |
| ability to inoculate microorganisms using basic inoculation techniques |
| teaching methods |
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| Knowledge |
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| E-learning |
| E-learning |
| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| Individual work of students |
| Individual work of students |
| Skills |
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| Practice exercises |
| Practice exercises |
| Simple experiments |
| Simple experiments |
| assessment methods |
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| Knowledge |
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| Analysis of another type of paper written by the student (Casuistry, diary, plan ...) |
| Analysis of another type of paper written by the student (Casuistry, diary, plan ...) |
| Preparation of a presentation, giving a presentation |
| Preparation of a presentation, giving a presentation |
| Systematic observation of the student |
| Systematic observation of the student |
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Recommended literature
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BÁLEŠ, V. a kol. Biochemické technológie: Biochemical Technologies. Bratislava: AB-ART, 2003. ISBN 80-89006-75-2.
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KOTRBA, P., BABŮREK, I., KREJZLÍK, Z. Návody ke cvičením z biologie. Praha: VŠCHT Praha, 2006. ISBN 80-7080-623-0.
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POLLACK, Robert A. Laboratory exercises in microbiology. 3rd ed.. Hoboken, NJ: John Wiley, 2009. ISBN 978-0-470-13392-7.
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SEIDMAN, L. Basic Laboratory Calculations for Biotechnology. San Francisco, Pearson, 2008. ISBN 978-0-13-223810-6.
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SEIDMAN, L., MOORE, C. J. Basic Laboratory Methods for Biotechnology, 2nd ed.. San Francisco, Pearson, 2009. ISBN 978-0-321-57014-7.
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ZAJONCOVÁ, L., TARKOWSKI, P. Základní chemické výpočty pro biochemiky a biotechnology. Olomouc: UP Olomouc, 2016. ISBN 9788024450025.
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