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Lecturer(s)
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Janalíková Magda, doc. Mgr. Ph.D.
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Bartošová Lucie, Ing.
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Koutný Marek, prof. Mgr. Ph.D.
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Baťová Adéla, Mgr.
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Course content
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1. Introduction to molecular biology. 2. Cell structure of living organisms. 3. Nucleic acids - types, structure, properties. 4. Proteins - structure, properties. 5. Genome of viruses, prokaryotic and eukaryotic organisms. 6. DNA replication. 7. Transcription. 8. Translation. 9. Changes in genetic information. 10. Mutation. 11. Molecular biology methods. 12. Isolation of nucleic acids, electrophoresis. 13. Amplification techniques. 14. Application of molecular biology methods.
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Learning activities and teaching methods
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- Preparation for examination
- 120 hours per semester
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| prerequisite |
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| Knowledge |
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| Knowledge of basics of biology and general microbiology. |
| Knowledge of basics of biology and general microbiology. |
| learning outcomes |
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| describe the basic structure of prokaryotic and eukaryotic cells, including the components of the genome |
| describe the basic structure of prokaryotic and eukaryotic cells, including the components of the genome |
| explain the basic structure of nucleic acids and proteins |
| explain the basic structure of nucleic acids and proteins |
| elucidate the transfer of genetic information in living cells (replication, transcription, translation) |
| elucidate the transfer of genetic information in living cells (replication, transcription, translation) |
| describe the basic methods of molecular biology |
| describe the basic methods of molecular biology |
| Skills |
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| apply various methods of DNA isolation, evaluates the results, and determine the quality, purity, and concentration of the obtained DNA |
| apply various methods of DNA isolation, evaluates the results, and determine the quality, purity, and concentration of the obtained DNA |
| perform agarose gel electrophoresis and interpret the obtained results |
| perform agarose gel electrophoresis and interpret the obtained results |
| design DNA amplification using the PCR method |
| design DNA amplification using the PCR method |
| use appropriate restriction enzymes depending on the intended DNA manipulation, perform restriction analysis, and evaluate the results |
| use appropriate restriction enzymes depending on the intended DNA manipulation, perform restriction analysis, and evaluate the results |
| analyze DNA sequencing results for the purpose of microbial identification |
| analyze DNA sequencing results for the purpose of microbial identification |
| teaching methods |
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| Knowledge |
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| Lecturing |
| Lecturing |
| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Skills |
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| Simple experiments |
| Simple experiments |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Didactic test |
| Didactic test |
| Analysis of works made by the student (Technical products) |
| Analysis of works made by the student (Technical products) |
| Written examination |
| Written examination |
| Grade (Using a grade system) |
| Grade (Using a grade system) |
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Recommended literature
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ALBERTS, B. Základy buněčné biologie. Ústí nad Labem: Espero, 2001. ISBN 80-902906-2-0.
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BROWN, T.A. Gene Cloning and DNA Analysis: An Introduction. 7th Ed.. Chichester: Wiley Blackwell, 2016. ISBN 978-1-119-07256-0.
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CRAIG, N.L. Molecular Biology: Principles of Genome Function. 2nd Ed.. Oxford University Press. ISBN 978-0-19-965857-2.
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ROSYPAL, S. úvod do molekulární biologie. Brno: Stanislav Rosypal, 2006. ISBN 80-092562-5-2.
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SNUSTAD, D.P., SIMMONS, M.J., RELICHOVÁ, J. Genetika. Brno: MU Brno, 2005. ISBN 978-80-210-4852-2.
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ŠMARDA, J. Metody molekulární biologie. Brno: MU Brno, 2005. ISBN 80-210-3841-1.
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