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Lecturer(s)
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Ingr Marek, doc. RNDr. Ph.D.
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Course content
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1. Determination of DNA concentration using quantitative PCR (qPCR). 2. Polyacrylamide gel electrophoresis of proteins in denaturing medium of sodium dodecyl sulphate (SDS PAGE). 3. Preparative gel chromatography of proteins. 4. Isolation of proteins using cation exchange chromatography. 5. Determination of acetate and chloride anions using capillary isotachophoresis. 6. Determination of vitamin C in citrus fruits using isocratic HPLC method. 7. Determination of vitamin B2 in animal products using gradient HPLC method. 8. Determination of ethanol in alcoholic beverages using gas chromatography. 9. Qualitative determination of volatile compounds in complex food matrix by HeadSpace-GC-MS method. 10. Preparative separation of the mixture using HPLC and characterization of isolated components.
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Learning activities and teaching methods
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Simple experiments, Practice exercises, Individual work of students
- Participation in classes
- 42 hours per semester
- Preparation for course credit
- 18 hours per semester
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| learning outcomes |
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| Knowledge |
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| explain the principle of the quantitative PCR method |
| explain the principle of the quantitative PCR method |
| explain the principle of preparative gel chromatography of proteins |
| explain the principle of preparative gel chromatography of proteins |
| explain the operation of gas chromatography |
| explain the operation of gas chromatography |
| describe the HeadSpace-GC-MS method |
| describe the HeadSpace-GC-MS method |
| explain the difference between isocratic and gradient HPLC methods |
| explain the difference between isocratic and gradient HPLC methods |
| Skills |
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| determine DNA concentration by quantitative PCR |
| determine DNA concentration by quantitative PCR |
| isolate proteins by cation-exchange chromatography |
| isolate proteins by cation-exchange chromatography |
| determination of ethanol in alcoholic beverages by gas chromatography |
| determination of ethanol in alcoholic beverages by gas chromatography |
| determination of acetate and chloride anions using capillary isotachophoresis |
| determination of acetate and chloride anions using capillary isotachophoresis |
| determination of vitamin C by isocratic HPLC |
| determination of vitamin C by isocratic HPLC |
| teaching methods |
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| Knowledge |
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| Individual work of students |
| Individual work of students |
| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| Skills |
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| Simple experiments |
| Simple experiments |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Analysis of the student's performance |
| Analysis of the student's performance |
| Analysis of works made by the student (Technical products) |
| Analysis of works made by the student (Technical products) |
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Recommended literature
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ATKINS, P.W. Atkins´ Physical Chemistry. 10th Ed. New York: Oxford University Press, 2014. ISBN 9780199697403.
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INGR, M., HALABALOVÁ, V. Návody k laboratorním cvičením ze separačních metod - interní text..
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Kodíček, M., Karpenko, V. Biofyzikální chemie. Praha, 1997.
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Nováková, L., Douša, M. Moderní HPLC separace v teorii a praxi I. Hradec Králové, Klatovy. ISBN 978-80-260-4244.
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Nováková, L., Douša, M. Moderní HPLC separace v teorii a praxi II.. Hradec Králové, Klatovy. ISBN 978-80-260-4244-0.
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Prosser V. a kol. Experimentální metody biofyziky. Praha, 1989. ISBN 80-244-0602-0.
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Štulík, Karel. Analytické separační metody. 1. vyd. Praha : Karolinum, 2004. ISBN 80-246-0852-9.
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