|
Lecturer(s)
|
-
Dastychová Lenka, RNDr. Ph.D.
-
Vícha Robert, doc. Mgr. Ph.D.
|
|
Course content
|
1. Basic principles and instrumentation of NMR. 2. Practical aspects of acquisition of NMR spectra. 3. Possibilities of using NMR spectroscopy (liquid and solid phase), MRI. 4. Qualitative 1H NMR. 5. Qualitative NMR of other nuclei 13C, 19F, 31P, 15N. 6. Quantitative NMR spectroscopy. 7. NMR in food analysis. 8. Use of NMR in authentication of food and food raw materials. 9. 2D homonuclear correlation experiments. 10. 2D heteronuclear correlation experiments. 11. DOESY spectroscopy. 12. Use of NMR in online monitoring of chemical transformations. 13. NMR techniques for monitoring chemical exchange. 14. NMR of the solid phase.
|
|
Learning activities and teaching methods
|
Monologic (Exposition, lecture, briefing), Simple experiments
- Participation in classes
- 56 hours per semester
- Home preparation for classes
- 20 hours per semester
- Preparation for course credit
- 14 hours per semester
- Preparation for examination
- 30 hours per semester
|
| learning outcomes |
|---|
| Knowledge |
|---|
| Areas of possible applications of NMR experiments in food analysis. |
| Areas of possible applications of NMR experiments in food analysis. |
| Areas of possible applications of NMR experiments in food analysis. |
| Areas of possible applications of NMR experiments in food analysis. |
| Uses and limits of basic 2D NMR experiments for structural analysis and identification of organic compounds. |
| Uses and limits of basic 2D NMR experiments for structural analysis and identification of organic compounds. |
| Use of 2H NMR spectroscopy. |
| Use of 2H NMR spectroscopy. |
| Skills |
|---|
| Identification of an unknown compound (constitution) based on 1H, 13C, HH-COSY, HX-HSQC, HX-HMBC experiments. |
| Identification of an unknown compound (constitution) based on 1H, 13C, HH-COSY, HX-HSQC, HX-HMBC experiments. |
| Configuration analysis of an unknown compound based on NOESY/ROESY experiments. |
| Configuration analysis of an unknown compound based on NOESY/ROESY experiments. |
| 21 / 5 000 Analysis of DOSY spectra. |
| 21 / 5 000 Analysis of DOSY spectra. |
| teaching methods |
|---|
| Knowledge |
|---|
| Monologic (Exposition, lecture, briefing) |
| Monologic (Exposition, lecture, briefing) |
| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Skills |
|---|
| Simple experiments |
| Simple experiments |
| Practice exercises |
| Practice exercises |
| assessment methods |
|---|
| Knowledge |
|---|
| Written examination |
| Written examination |
| Oral examination |
| Grade (Using a grade system) |
| Grade (Using a grade system) |
| Oral examination |
|
Recommended literature
|
-
BERGER, S., BRAUN, S. 200 and More NMR Experiments: A Practical Course. 3rd Ed.. Wiley-VCh Verlag, 2004. ISBN 978-3527310678.
-
FIELD, L.D., LI, H.L., MAGILL, A.M. Instructor's Guide and Solutions Manual to Organic Structures from 2D NMR Spectra. John Wiley and Sons Ltd., 2015. ISBN 978-1119027256.
-
HORE, P. NMR: The Toolkit. How Pulse Sequences Work. 2nd Ed.. Oxford University Press, 2015. ISBN 978- 0198703426.
-
IGGO, J.A. NMR Spectroscopy in Inorganic Chemistry. Oxford University Press, 2000. ISBN 978-0198558903.
-
JACOBSEN, N.E. NMR Data Interpretation Explained: Understanding 1D and 2D NMR Spectra of Organic Compounds and Natural Products. 1st Ed.. Hoboken, New Jersey: John Wiley & Sons, Inc., 2016. ISBN 978-1118370223.
-
KEELER, J. Understanding NMR Spectroscopy. John Wiley and Sons Ltd., 2002. ISBN 978-0470746080.
-
Martin Dračínský. NMR spektroskopie pro chemiky. Praha, 2021. ISBN 978-80-7444-085-4.
-
SPYROS, A., DAIS, P. NMR Spectroscopy in Food Analysis: RSC (RSC Food Analysis Monographs, Book 10).. Cambridge: The Royal Society of Chemistry, 2013. ISBN 978-1849731751.
|