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Lecturer(s)
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Course content
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The subject includes the following thematic areas: 1. History of biomolecule production and introduction to cell technologies 2. Cell cultures and cell lines 3. Culture media 4. Cultivation conditions (sera, vitamins, and supplements in culture media) 5. Biomolecules as growth supplements 6. Growth factors influencing biomolecule production 7. Bacterial and yeast cultures 8. Insect and plant cell cultures 9. Cellular products and biomolecule production simulation 10. Separation, regeneration, and purification (e.g., PHA, biosurfactants) 11. Bioreactors - kinetics, modeling, and growth dynamics of biomolecule producers 12. In vitro simulation 13. Applications from laboratory to pilot-scale production 14. 3D cell printing in polysaccharide-based gels
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Learning activities and teaching methods
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Lecturing, Monologic (Exposition, lecture, briefing), Simple experiments
- Participation in classes
- 20 hours per semester
- Home preparation for classes
- 8 hours per semester
- Preparation for course credit
- 24 hours per semester
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| prerequisite |
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| Knowledge |
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| To define the basic terms in microbiology, particularly sterilization, disinfection, contamination, and related. |
| To define the basic terms in microbiology, particularly sterilization, disinfection, contamination, and related. |
| Skills |
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| Cultivate various types of cell cultures, including bacteria, yeasts, insect, and plant cells |
| Cultivate various types of cell cultures, including bacteria, yeasts, insect, and plant cells |
| Separate, regenerate, and purify biomolecular substances. |
| Separate, regenerate, and purify biomolecular substances. |
| Model and analyze the kinetics and dynamics of biomolecule-producing organisms in bioreactors. |
| Model and analyze the kinetics and dynamics of biomolecule-producing organisms in bioreactors. |
| Gain practical experience in the production of biosurfactants. |
| Gain practical experience in the production of biosurfactants. |
| learning outcomes |
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| Knowledge |
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| To achieve professional knowledge, the following teaching methods are used: Lecturing Demonstration Dialogic methods (discussion, conversation, brainstorming) |
| To achieve professional knowledge, the following teaching methods are used: Lecturing Demonstration Dialogic methods (discussion, conversation, brainstorming) |
| teaching methods |
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| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Monologic (Exposition, lecture, briefing) |
| Educational trip |
| Educational trip |
| Monologic (Exposition, lecture, briefing) |
| Skills |
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| Simple experiments |
| Simple experiments |
| Educational trip |
| Educational trip |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Didactic test |
| Analysis of works made by the student (Technical products) |
| Analysis of works made by the student (Technical products) |
| Didactic test |
| Grade (Using a grade system) |
| Grade (Using a grade system) |
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Recommended literature
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DŁUGONSKI. Microbial Biotechnology in the Laboratory and Practice: Theory, Exercises, and Specialist Laboratories. Łódź?Kraków. 2023.
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Hochfeld. Producing Biomolecular Substances with Fermenters, Bioreactors, and Biomolecular Synthesizers. Boca Raton. 2006.
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SINGH. Bioreactors: Sustainable Design and Industrial Applications in Mitigation of GHG Emissions. Amsterdam. 2020.
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SINGH, H.B., VAISHNAV, A. New and Future Developments in Microbial Biotechnology and Bioengineering: Sustainable Agriculture: Revisiting Green Chemicals. Amsterdam. 2022.
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SINGH, S.P., UPADHYAY, S.K. Microbial Bioreactors for Industrial Molecules. Hoboken. 2023.
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SINGH, V. Microbial Cell Factories Engineering for Production of Biomolecules. Waltham. 2021.
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