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Lecturer(s)
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Jančová Petra, Mgr. Ph.D.
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Riemel Jakub, Ing.
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Buňková Leona, prof. RNDr. Ph.D.
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Course content
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Overview of methods used in microbiology. Sampling and processing of samples for microbiological analysis. Conventional microbiological methods - culture methods. Conventional microbiological methods - microscopic methods. Methods for identifying microorganisms. Molecular biological methods used in microbiology - PCR, hybridization. Molecular biological methods used in microbiology - sequencing, genome analysis, bioinformatics. Immunochemical methods for determining microorganisms - precipitation methods; non-precipitation methods with a label; non-precipitation methods without a label. Methods used in clinical microbiology. Instrumental methods used in microbiology - spectrometric, chromatographic, electromigration methods; flow cytometry. Susceptibility of microorganisms to antimicrobial agents - disk diffusion method, dilution tests, genotyping. Determination of metabolites of microorganisms - proteins and their derivatives. Determination of metabolites of microorganisms - carbohydrates, lipids and their derivatives. Important microbial toxins and methods for their determination. Determination of other secondary metabolites of microorganisms - pigments, antibiotics, vitamins and others.
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Learning activities and teaching methods
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Lecturing, Dialogic (Discussion, conversation, brainstorming), Methods for working with texts (Textbook, book), Simple experiments, Teamwork, Analysis of a presentation, Individual work of students, Students working in pairs
- Home preparation for classes
- 14 hours per semester
- Preparation for course credit
- 25 hours per semester
- Preparation for examination
- 25 hours per semester
- Participation in classes
- 56 hours per semester
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| prerequisite |
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| Knowledge |
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| Knowledge of general microbiology. |
| Knowledge of general microbiology. |
| Skills |
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| Work in a (micro)biological laboratory. |
| Work in a (micro)biological laboratory. |
| learning outcomes |
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| Knowledge |
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| explain the specifics of working with microorganisms and biological material |
| explain the specifics of working with microorganisms and biological material |
| describe and explain the individual steps of microbiological analysis |
| describe and explain the individual steps of microbiological analysis |
| describe and explain the differences between conventional and modern microbiological methods |
| describe and explain the differences between conventional and modern microbiological methods |
| explain the principle of methods for determining the sensitivity of microorganisms to antimicrobial agents and their use |
| explain the principle of methods for determining the sensitivity of microorganisms to antimicrobial agents and their use |
| list and characterize examples of methods for determining the main microbial metabolites and toxins |
| list and characterize examples of methods for determining the main microbial metabolites and toxins |
| Skills |
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| apply principles of working with microorganisms and biological material |
| apply principles of working with microorganisms and biological material |
| propose appropriate methods for analyzing the sample |
| propose appropriate methods for analyzing the sample |
| perform standard methods of isolation of selected sample components for their subsequent determination |
| perform standard methods of isolation of selected sample components for their subsequent determination |
| operate laboratory equipment |
| operate laboratory equipment |
| apply conventional and modern microbiological methods (cultivation, microscopy, molecular biology methods, instrumental methods) in practice |
| apply conventional and modern microbiological methods (cultivation, microscopy, molecular biology methods, instrumental methods) in practice |
| teaching methods |
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| Knowledge |
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| Lecturing |
| Lecturing |
| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Simple experiments |
| Simple experiments |
| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| Individual work of students |
| Individual work of students |
| Skills |
|---|
| Simple experiments |
| Simple experiments |
| Individual work of students |
| Individual work of students |
| Students working in pairs |
| Students working in pairs |
| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Observation |
| Observation |
| Practice exercises |
| Practice exercises |
| Analysis of a presentation |
| Analysis of a presentation |
| Demonstration |
| Demonstration |
| Teamwork |
| Teamwork |
| assessment methods |
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| Knowledge |
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| Analysis of works made by the student (Technical products) |
| Grade (Using a grade system) |
| Grade (Using a grade system) |
| Analysis of works made by the student (Technical products) |
| Composite examination (Written part + oral part) |
| Composite examination (Written part + oral part) |
| Didactic test |
| Didactic test |
| Written examination |
| Written examination |
| Preparation of a presentation |
| Preparation of a presentation |
| Preparation of a presentation, giving a presentation |
| Preparation of a presentation, giving a presentation |
| Analysis of works made by the student (Technical products) |
| Analysis of works made by the student (Technical products) |
| Systematic observation of the student |
| Systematic observation of the student |
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Recommended literature
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Bisen P.S. a Sharma A. Introduction to Instrumentation in Life Sciences. Boca Raten, CRC Press. 2012.
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Bursová Š a kol. Mikrobiologické laboratorní metody. Brno, VFU. 2014.
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Hodek, P., Páca, J., & Šulc, M. Laboratorní cvičení z biologie a biochemie mikroorganismů. Praha: Karolinum, 2009.
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Kolářová L. Obecná a klinická mikrobiologie. Praha. 2020.
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Leboffe M.J. a Pierce B.E. Microbiology: Laboratory Theory & Application. 3rd Ed. Englewood. 2010.
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Levin R.E. Rapid detection and characterization of foodborne pathogens by molecular techniques. 2010.
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Melter O. a Malmgren A. Principy a praktika lékařské mikrobiologie. Praha. 2014.
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Votava M. Lékařská mikrobiologie ? vyšetřovací metody. Brno. 2010.
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