|
Lecturer(s)
|
-
Janalíková Magda, doc. Mgr. Ph.D.
-
Baťová Adéla, Mgr.
-
Koutný Marek, prof. Mgr. Ph.D.
|
|
Course content
|
- Basic concepts, definitions, importance and perspectives. - Methods of DNA isolation, purification and manipulation. - DNA cloning, amplification techniques, their applications. - Molecular analysis of the genome. Gene libraries. - Mutagenesis - possibilities, variants, significance. - General characteristics of vectors for gene transfer to prokaryotes and eukaryotes, expression systems. - Gene cloning, gene engineering. - Genetically modified organisms, transgenesis - definition, legislation, use. - Gene therapies. - Application of recombinant DNA methods in food, agriculture, biotechnology, industry and healthcare.
|
|
Learning activities and teaching methods
|
Monologic (Exposition, lecture, briefing), Dialogic (Discussion, conversation, brainstorming), Practice exercises
- Preparation for examination
- 60 hours per semester
- Participation in classes
- 12 hours per semester
- Preparation for course credit
- 10 hours per semester
- Home preparation for classes
- 68 hours per semester
|
| learning outcomes |
|---|
| Knowledge |
|---|
| describe the fundamental concepts in the field of recombinant technologies |
| describe the fundamental concepts in the field of recombinant technologies |
| explain mutagenesis, genome analysis, and the principle of gene libraries |
| explain mutagenesis, genome analysis, and the principle of gene libraries |
| define genetically modified organisms and the principle of cloning |
| define genetically modified organisms and the principle of cloning |
| clarify the use of vectors in transgenesis in prokaryotes and eukaryotes |
| clarify the use of vectors in transgenesis in prokaryotes and eukaryotes |
| provide examples of the application of recombinant DNA methods in food industry, agriculture, biotechnology, and healthcare |
| provide examples of the application of recombinant DNA methods in food industry, agriculture, biotechnology, and healthcare |
| Skills |
|---|
| find a scientific article on recombinant technologies using scientific databases |
| find a scientific article on recombinant technologies using scientific databases |
| search for a method of DNA manipulation in the scientific article |
| search for a method of DNA manipulation in the scientific article |
| explain a specific method used within the scope of the scientific study |
| explain a specific method used within the scope of the scientific study |
| distinguish amplification techniques and recombinant DNA techniques |
| distinguish amplification techniques and recombinant DNA techniques |
| justify the use of recombinant DNA techniques within a specific scientific study |
| justify the use of recombinant DNA techniques within a specific scientific study |
| teaching methods |
|---|
| Knowledge |
|---|
| Educational trip |
| Educational trip |
| Monologic (Exposition, lecture, briefing) |
| Monologic (Exposition, lecture, briefing) |
| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| Skills |
|---|
| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Educational trip |
| Educational trip |
| Practice exercises |
| Practice exercises |
| Individual work of students |
| Individual work of students |
| assessment methods |
|---|
| Knowledge |
|---|
| Didactic test |
| Written examination |
| Analysis of seminar paper |
| Didactic test |
| Oral examination |
| Oral examination |
| Written examination |
| Analysis of seminar paper |
|
Recommended literature
|
-
ALBERTS, B. Základy buněčné biologie. Ústí nad Labem: Espero, 2001. ISBN 80-902906-2-0.
-
BROWN, T.A. Gene Cloning and DNA Analysis: An Introduction. 7th Ed.. Chichester: Wiley Blackwell, 2016. ISBN 978-1-119-07256-0.
-
CRAIG, N.L. Molecular Biology: Principles of Genome Function. 2nd ed.. Oxford: Oxford University Press. ISBN 978-0-19-965857-2.
-
DALE, J., SCHANTZ, M., PLANT, N. From genes to genomes: concepts and applications of DNA technology, 3rd ed.. Chichester, West Sussex: Wiley-Blackwell, 2012. ISBN 978-0-470-68385-9.
-
ROSYPAL, S., DOŠKAŘ, J. Úvod do molekulární biologie. 3. díl., 2. rozš. vyd.. Brno: Stanislav Rosypal, 1997.
-
RUML, T., RUMLOVÁ, M., PAČES, V. Genové inženýrství. VŠCHT, Praha, 2012.
|