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Lecturer(s)
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Jančová Petra, Mgr. Ph.D.
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Pleva Pavel, Ing. Ph.D.
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Píšová Pavlína, Ing.
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Course content
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1. Introductory lesson: Laboratory work safety training, weighing, pipetting, measuring with UV / VIS spectrophotometer, buffer preparation. 2. Carbohydrates: The chemical properties of carbohydrates, monosaccharides group reactions, identification of the unknown sample, hydrolysis of polysaccharide. 3. Amino acids, proteins: Reactions of amino acid side chains, the identification of the unknown sample, the denaturation of proteins. 4. Afinity chromatography. 5. Enzymes, enzyme activity: Monitoring and quantification amylase activity of human saliva, proof of peroxidase activity in milk. 6. Purification of an enzyme: Two-step purification of phenoloxidases from fruiting bodies of mushrooms, acetone fractionation, ion exchange chromatography, activity detection. 7. Lipids: Isolation of phospholipids from egg yolk; evidence reactions (cholesterol, choline, phosphoric acid); emulsifying ability of phosphatides.
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Learning activities and teaching methods
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Simple experiments
- Participation in classes
- 8 hours per semester
- Home preparation for classes
- 30 hours per semester
- Preparation for course credit
- 22 hours per semester
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| prerequisite |
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| Knowledge |
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| Knowledge of organic chemistry and biochemistry. |
| Knowledge of organic chemistry and biochemistry. |
| learning outcomes |
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| Skills |
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| have practical experience with basic operations in a biochemical laboratory. |
| have practical experience with basic operations in a biochemical laboratory. |
| know to prepare buffers and work in a buffered environment. |
| know to prepare buffers and work in a buffered environment. |
| know the basics of working with proteins and enzymes. |
| know the basics of working with proteins and enzymes. |
| know the basics of protein purification. |
| know the basics of protein purification. |
| teaching methods |
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| Knowledge |
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| Educational trip |
| Educational trip |
| Methods for working with texts (Textbook, book) |
| Methods for working with texts (Textbook, book) |
| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Skills |
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| Simple experiments |
| Simple experiments |
| Educational trip |
| Educational trip |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Analysis of another type of paper written by the student (Casuistry, diary, plan ...) |
| Analysis of another type of paper written by the student (Casuistry, diary, plan ...) |
| Grade (Using a grade system) |
| Grade (Using a grade system) |
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Recommended literature
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KODÍČEK M., VALENTOVÁ O., HYNEK R. Biochemie; chemický pohled na biologický svět. Praha: VŠCHT v Praze, 2015. ISBN 978-80-7080-927-3.
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Robert K. Murray. Harperova ilustrovaná biochemie. Galén, spol. s r.o., 2012. ISBN 978-80-7262-907-7.
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