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Lecturer(s)
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Filip Jaroslav, doc. Ing. Ph.D.
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Course content
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- Introduction to instrumental analysis, preparation of samples for analysis. - Data processing and evaluation, extraction, SPE. - Electrochemical methods (potentiometry, polarography, voltammetry, conductometry, coulometry). - Electromigration methods (electrophoresis, isotachoforesis, isoelectric focusing). - Introduction to spectral methods, AES. - AAS, XRF. - UV-VIS, fluorescence spectrometry. - IR, Raman spectroscopy. - NMR and nephelometry, turbidimetry, Refractometry, Interferometry. - Mass Spectrometry (MS). - Introduction to chromatography, TLC. - HPLC, UPLC, GPC. - IEC, affinity chromatography and chiral GC. - SFC, quantitative and qualitative chromatography.
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Learning activities and teaching methods
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Lecturing, Monologic (Exposition, lecture, briefing)
- Preparation for examination
- 90 hours per semester
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| prerequisite |
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| Knowledge |
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| The knowledge of inorganic, organic, analytical chemistry and work safety. |
| The knowledge of inorganic, organic, analytical chemistry and work safety. |
| learning outcomes |
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| Basic knowledge about sample acquiring, storage and pretreatment |
| Basic knowledge about sample acquiring, storage and pretreatment |
| Basic principles of data evaluation - statistical evaluation and calibration methods |
| Basic principles of data evaluation - statistical evaluation and calibration methods |
| Basic knowledge of principles, pros and cons and applications of electrochemical analytical methods |
| Basic knowledge of principles, pros and cons and applications of electrochemical analytical methods |
| Basic knowledge of principles, pros and cons and applications of spectroscopic analytical methods |
| Basic knowledge of principles, pros and cons and applications of spectroscopic analytical methods |
| Basic knowledge of principles, pros and cons and applications of chromatographic methods |
| Basic knowledge of principles, pros and cons and applications of chromatographic methods |
| Skills |
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| Suggest an appropriate sampling method and sample treatment for a given assessed material, regarding its properties |
| Suggest an appropriate sampling method and sample treatment for a given assessed material, regarding its properties |
| Apply the basic statistic tools on analysis outputs and create a calibration curve |
| Apply the basic statistic tools on analysis outputs and create a calibration curve |
| Decide which electroanalytical method can be used for a given analytical task and give reasons for the decision |
| Decide which electroanalytical method can be used for a given analytical task and give reasons for the decision |
| Decide which spectroscopic method can be used for a given analytical task and give reasons for the decision |
| Decide which spectroscopic method can be used for a given analytical task and give reasons for the decision |
| Decide which chromatographic method can be used for a given analytical task and give reasons for the decision |
| Decide which chromatographic method can be used for a given analytical task and give reasons for the decision |
| teaching methods |
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| Knowledge |
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| Lecturing |
| Lecturing |
| Monologic (Exposition, lecture, briefing) |
| Monologic (Exposition, lecture, briefing) |
| Skills |
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| Dialogic (Discussion, conversation, brainstorming) |
| Dialogic (Discussion, conversation, brainstorming) |
| Monologic (Exposition, lecture, briefing) |
| Monologic (Exposition, lecture, briefing) |
| Practice exercises |
| Practice exercises |
| assessment methods |
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| Knowledge |
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| Composite examination (Written part + oral part) |
| Composite examination (Written part + oral part) |
| Grade (Using a grade system) |
| Grade (Using a grade system) |
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Recommended literature
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Holzbecher, Z., Churáček, J. Analytická chemie. Praha : SNTL, 1987.
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Rubinson, Kenneth A. Contemporary instrumental analysis. Upper Saddle River : Prentice Hall, 2000. ISBN 137907265.
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Willard, H. H. Instrumental methods of analysis. Belmont : Wadsworth Publishing, 1988. ISBN 0534081428.
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